PEX-01-03 - Lab report exorcise 1 -3 Physiology lab 9.1 PDF

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Lab report exorcise 1 -3 Physiology lab 9.1...

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Name: evelyn Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results You scored 75% by answering 3 out of 4 questions correctly. 1. Which of the following is true of osmosis? Your answer : d. It refers to the movement of solutes. Correct answer: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.

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Experiment Results Predict Question: Predict Question 1: What effect do you think increasing the Na+ Cl- concentration will have? Your answer : a. increased osmotic pressure Predict Question 2: What do you think will be the pressure result of the current experimental conditions? Your answer : c. pressure above the left beaker Stop & Think Questions: Why do you think there is no pressure change? You correctly answered: b. Sodium is able to diffuse through the pores. Why do you think there was no pressure change? You correctly answered: c. There is no net movement of water because the solute concentrations are the same. Experiment Data: Run Number 1 1 1

Solute Na+ ClAlbumin Glucose

MWCO 200 200 200

Start Conc. L 5.00 0.00 0.00

Pres. L 0 0 0

Start Conc. R 0.00 0.00 0.00

2 2 2 3 3

Na+ ClAlbumin Glucose Na+ ClAlbumin

200 200 200 50 50

10.00 0.00 0.00 10.00 0.00

0 0 0 0 0

0.00 0.00 0.00 0.00 0.00

0 0 0 0 0

0.0167 0.0000 0.0000 0.0167 0.0000

3 4 4 4 5 5

Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin

50 100 100 100 100 100

0.00 0.00 0.00 8.00 0.00 0.00

0 136 136 136 0 0

0.00 0.00 0.00 0.00 0.00 0.00

0 0 0 0 0 0

0.0000 0.0000 0.0000 0.0000 0.0000 0.0000

5 6 6 6 7 7

Glucose Na+ ClAlbumin Glucose Na+ ClAlbumin

100 200 200 200 200 200

8.00 0.00 0.00 8.00 0.00 9.00

0 0 0 0 153 153

8.00 0.00 0.00 0.00 0.00 0.00

0 0 0 0 0 0

0.0000 0.0000 0.0000 0.0036 0.0000 0.0000

7

Glucose

200

0.00

153

10.00

0

0.0044

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Pres. R 0 0 0

Rate 0.0093 0.0000 0.0000

Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. If you double the concentration of a nondiffusible solute that is on one side of a membrane, the osmotic pressure will You correctly answered: c. double. 2. Which membrane did not allow Na+ Cl- to pass through? You correctly answered: a. 20 MWCO 3. When a solute is able to diffuse through a membrane You correctly answered: b. equilibrium is reached. 4. Water diffuses You correctly answered: c. toward solutes.

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Review Sheet Results 1. Explain the effect that increasing the Na+ Cl- concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? Your answer: If is increasing the Na+Cl- the result will increase the osmotic pressure. Cause the more solute added the more water need to pass through

2. Describe one way in which osmosis is similar to simple diffusion and one way in which it is different. Your answer: Both of diffusion are passive transport of high concentration to low concentration. in the process osmosis differs from simple diffusion cause osmosis diffusion is the diffusion of water through a selective permeable membrane. 3. Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.” Your answer: this means that as a solute is increasing and the number of milliosmoles can rise, the water will decrease. Because water moves toward high concentration of solute. 4. The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction? Your answer: Glucose diffuses from right beaker to the left beaker until equilibrium is reached. Albumin can't diffuse through the membrane cause of its size.

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