Quiz - Chapter 11 PDF

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stion 1

Suppose you would like to examine a gene for the presence of introns, and you decide to use the hybridization experiment approach. Which of the following is a false statement concerning the hybridization experiment approach? Question options:

a) The mature mRNA for the gene of interest is isolated.

b) The coding DNA strand forms R loops during the experiment.

c) The mature mRNA is hybridized first to the coding DNA strand.

d) The DNA containing the gene of interest is denatured.

e) Introns within the DNA form loops during the experiment. 1/ po

estion

Suppose you would like to examine a gene for the presence of introns. If you use the approach where you compare gDNA to cDNA, _____________ will be used to convert the mature mRNA into cDNA. Question options:

Poly-A polymerase

Reverse transcriptase

U1 snRNP

RNA 5’-triphosphatase

CPSF 1/ poi

estion

Which of the following involves catalytic RNA molecules? Question options:

a) 5’ capping

b) Converting mature mRNA to cDNA

c) 3’ polyadenylation

d) Group I intron splicing mechanism

e) Identifying introns by the hybridization experiment. 1/ po

estion

The group I intron splicing mechanism involves ____________, while the group II intron splicing mechanism involves _____________. Question options:

a) a cytidine nucleoside; an adenosine nucleoside

b) a thymidine nucleoside; a guanosine nucleoside

c) an adenosine nucleoside; a guanine nucleotide within the intron

d) a guanosine nucleoside; an adenine nucleotide within the intron

e) a uridine nucleoside; the formation of a lariat 1/ po

estion

The spliceosome Question options:

a) is composed of subunits called snRNPs.

b) contains ribozyme components.

c) recognizes introns that contain a 5’-splice site, a branch site, and a 3’ splice site.

d) is found in eukaryotes only.

e) All of the above are true of the spliceosome.

f) None of the above is true of the spliceosome. 1/ po

estion

_______________ is an example of an endonuclease. Question options:

a) Guanylyltransferase

b) Reverse transcriptase

c) CPSF

d) Methyltransferase

e) Poly-A polymerase

f) RNA 5’-triphosphatase 1/ po

estion

Molecular components called U1, U2, U4, U5, and U6 function during Question options:

a) the Group I intron splicing mechanism.

b) the spliceosome splicing mechanism.

c) 5’ capping.

d) 3’ polyadenylation.

e) the Group II intron splicing mechanism. 1/ po

estion

Which of the following is not a function of the 5’ cap? Question options:

a) The 5’ cap serves to protect the pre-mRNA from exonuclease attack.

b) The 5’ cap serves as a transport signal to move the mature mRNA to the cytoplasm of the cell.

c) The 5’ cap serves to terminate transcription by releasing the pre-mRNA from RNA polymerase II.

d) The 5’ cap serves as a binding site for protein factors that initiate translation.

e) The 5’ cap serves to regulate RNA splicing. 1/ po

estion

________________ involves guanylyltransferase. Question options:

a) The spliceosome splicing mechanism

b) The Group I intron splicing mechanism

c) 3’ polyadenylation

d) The Group II intron splicing mechanism

e) 5’ capping 1/ po

estion

________________ recognizes a 5’-AAUAAA-3’ sequence within the pre-mRNA. Question options:

a) Reverse transcriptase

b) RNA 5’-triphosphatase

c) A snRNP

d) Poly-A polymerase

e) CPSF...