UNIT TASK #16 Mounting and Labelling PDF

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NA13 MICROTOMT SECTIONIH...

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Unit task #16: Mounting and Labelling

Critical Thinking Questions: Answer the following. Submit it on a .docx document. 1.

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What is the importance of mounting tissue slides? (2 pts.) - The importance of mounting tissue slides is that essential for preserving the specimen during storage as well as for enhancing imaging quality during microscopy What is the purpose of Sugar and Glycerol in Aqueous Mounting Medium? (3 pts.) - The purpose of Sugar is that increases refractive index and the importance of Glycerol is to prevent drying and cracking. What is the refractive index of Glycerin? ( 1 pt.) - Refractive index of 1.46 What is the refractive index of Farrant’s Medium? (1. pt.) - Refractive Index 1.43 What is the most commonly used Resinous Mounting Medium? Why? (5 pts.) - The most commonly used resinous mounting medium is the DPX Dibutylphthalate Polystyrene Xylene, because of its ability to preserve stains, and dry quickly. Explain the ringing of tissue slides. (3 pts.) - The Ringing is the process of sealing the margins of the coverslip to prevent the escape of fluid or semi-fluid mounts and evaporation of mountant, to fix the coverslip in place, and to prevent sticking of the slides upon storage. - The term “ringing” originated because round coverslips were initially used and the coating applied in the form of a circle or “ring.” A liquid preparation sealed well with nail polish could last some months. - The ringing media used may be Kronig cement made up of two parts paraffin wax mixed with 4-9 parts powdered colophonium resin, heated and filtered. Also available are cellulose adhesives such as Durofix....