Bio intro - stefan milenkovic PDF

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Introduction ! Auxenochlorella protothecoides is a heterotrophic unicellular"green microalgae"in the class"Trebouxiophyta"known for its potential application in"biofuel"production (Polat, Altınbaş, 2020) . Algae have gotten a lot of attention in recent years for biofuel production due to their faster growth rate, higher lipid content, and lower effect on food security than other agriculture-based feedstocks (eXiao, eLu, eDai, eWu, 2015). These characteristics make A. protothecoides an ideal microorganism for testing the effect of glucose concentration on growth rate. Microalgae growth is regulated by a set of specific conditions that can be found in a wide range of environments. Chemical factors, such as glucose concentration, and physical factors such as temperature, light intensity and pH, are two types of factors that influence microalgae development (Xiao, Guo, Zhu, Muhammad, Deng, Hu, Wu, 2020). Glucose concentration is a critical factor in the growth of all organisms since it influences metabolic processes that are needed for cell maintenance and survival. (Krzemińska, Oleszek, 2016). Hence, the aim of this experiment is to establish the optimal conditions for maximising the growth of a particular microalgae, A.protothecoides by observing the effect of varying glucose concentration on the organism's growth rate. ! References Polat, E., & Altınbaş, M. (2020). Optimization of Auxenochlorella protothecoides lipid content using response surface methodology for biofuel production. Biomass Conversion and Biorefinery: Processing of Biogenic Material for Energy and Chemistry, 1. https://doi.org/10.1007/ s13399-020-00798-8! Yibo eXiao, Yue eLu, Junbiao eDai, & Qingyu eWu. (2015). Industrial fermentation of Auxenochlorella protothecoides for production of biodiesel and its application in vehicle diesel engines. Frontiers in Bioengineering and Biotechnology, 3. https://doi.org/10.3389/fbioe. 2015.00164! Yibo Xiao, Jianying Guo, Huachang Zhu, Anwar Muhammad, Haiteng Deng, Zhangli Hu, & Qingyu Wu. (2020). Inhibition of glucose assimilation in Auxenochlorella protothecoides by light. Biotechnology for Biofuels, 13(1), 1–13. https://doi.org/10.1186/s13068-020-01787-9! Krzemińska, I., & Marta Oleszek. (2016). Glucose supplementation-induced changes in the Auxenochlorella protothecoides fatty acid composition suitable for biodiesel production. Bioresource Technology, 218, 1294–1297. https://doi.org/10.1016/j.biortech.2016.07.104!...