EXSC322-2021-Lab 1 ONE NOTES PDF

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EXSC322, Semester 2, 2021-Lab 1- Exercise in the Heat Background Exercise in hot conditions presents athletes with numerous physiological challenges. In this laboratory you will learn to simulate hot conditions and measure changes in three key physiological variables to understand the effect of the hot environment on athletic performance: heart rate, hematocrit, and fluid loss. Hematocrit Whole blood has two major components, solid and liquid. The solid component is made up of cells including red and white blood cells. The liquid component is blood plasma, a yellowish fluid primarily consisting of water, salts and small proteins. The determination of haematocrit (Hct) is a process where these two components of blood are split apart via centrifugation of whole blood. The heavier solids are spun towards the outside of the spinning circular centrifuge leaving the fluid in the inner part of the tube. Hence the proportion of the two components can be determined by measuring the lengths of the 2 parts. In general, the normal Hct range for men is approximately 41 – 50% and for females 36 – 44%. During exercise sweat rate increases resulting in a decrease in central blood volume and plasma volume. Exercise Scientists assess Hct to determine dehydration status, fluid shifts during exercise, possible anaemia, changes in plasma volume with training and heat acclimation. In this tute you will be measuring Hct on individuals before and after exercise. Equipment needed HR monitor, centrifuge, capillary tubes, lancets, gloves, tissues, alcohol swabs, scales, bike. Procedures 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Have the participant sit quietly and make sure their fingers are warm. Take a finger and prepare with an alcohol swab. Use a lancet to prick a fingertip. Take a capillary tube and place near the drop of blood and wait until the tube is ¾ full. Plug the end of the tube with plasticine Place in the centrifuge and turn on for at least 3 minutes. Take out the tube and place in the Hct measurement tool. Make sure the line up the top and bottom levels of the blood in the tube with the lines indicated on the measurement tool. Adjust the measuring lever until it is exactly on the junction between the clear fluid and the red cells then read off the percentage on the scale. Weight the person in minimal clothing. Adorn Heart rate monitor and heat suit. Repeat the measurement after a 20min exercise bout, at approximately 70% HRmax (take 510 minutes initially to determine the correct intensity before putting on the heat suit). Reweigh the person (subtract the weight of any water consumed). Calculate sweat loss.

Condition Pre-exercise Post-exercise

Body weight

Results Time (min) 2 4 6 8 10 12 14 16 18 20

Heart rate

Graph your heart rates below and label axes appropriately

Haematocrit...