LQB180 HSE Assignment 2020 PDF

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Faculty of Health

LQB180 Foundations of Biochemistry

Introduction to Health, Safety & Environment (HSE) in the Biochemistry Laboratory YOUR COMPLETED ASSIGNMENT NEEDS TO BE PREPARED AS A DIGITAL DOCUMENT (using software programs such as Microsoft Office). YOUR COMPLETED ASSIGNMENT MUST BE SUBMITTED IN A DIGITAL FORMAT (MS Word documents or Adobe pdf documents are acceptable) USING TURNITIN ASSIGNMENT

ASSESSMENT DUE DATE = THURSDAY 19th MARCH, 2020

Student name: Student ID No: Date:

Why do you need to know about Health, Safety & Environment (HSE) in the laboratory? When working in a laboratory (and similar workplaces) it is important to be familiar with the health and safety rules and laboratory safety features that have been put in place for the health and safety of all workers and users of the space, as well as visitors to the workplace. Compliance with these safety regulations and effective implementation of safe working practices will ensure you develop an effective understanding of safe laboratory work practices and are able to apply these practices appropriately. Compliance is MANDATORY; it is an occupational health and safety (OHS) requirement at QUT, as well as being a significant part of standard good laboratory practices for any type of laboratory work in the biomedical science discipline areas (such as anatomy, biochemistry, cell & molecular biology, microbiology, and physiology).

Working Safely in the Laboratory – HSE Assignment

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Faculty of Health

LQB180 Foundations of Biochemistry

Learning objectives After engaging with this activity, you will demonstrate evidence of the ability to: • describe laboratory safety features, understand the reasons for safety rules and regulations in laboratory practice, • •

recognise Safety Data Sheets (SDS) and understand their importance for the safe use and handling of chemicals and related reagents or substances, develop an understanding of how to use SDS information to identify risk and safety phrases and formulate a Risk Assessment related to a specific chemical / solution used in biochemistry laboratories.

1. Laboratory Health, Safety & Environment Induction To help explain these Health, Safety and Environment (HSE) rules and procedures students are required to complete a Laboratory Health, Safety and Environment Induction. This online system requires students to watch an induction video, review a Faculty of Health HSE Guidelines document and complete an induction quiz. The video and guidelines document include rules and information about safe work practices for students in Q Block laboratories at Gardens Point incorporating: • Fire safety • Personal health and security • • • •

General laboratory safety Chemical safety Biological safety Discard safe handling procedures

The Faculty of Health Student Health Safety and Environment (HSE) Induction and mandatory safety quiz are available at http://qut.to/labsafety

It is a requirement that all students working in the labs in Q-Block complete the online Induction process by watching the video / reading the Q Block HSE manual, taking note of the rules and regulations highlighted in the manual, and then COMPLETING the quiz. THIS TASK MUST BE COMPLETED BEFORE STARTING PRACTICAL CLASSES Note: Successful completion of the Faculty of Health HSE induction requires an induction quiz score of 10 out of 10.

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LQB180 Foundations of Biochemistry

Successful completion of the induction with a quiz score of 10/10 by Friday 6th March (end of week 2) will be awarded marks (2 marks) that will contribute to this LQB180 HSE assessment.  Non-completion of the induction by the required date OR quiz scores of ≤9 will gain 0 marks.  You must print (or scan in) a copy of your certificate of completion and attach the print off / image to your assignment.  NO CERTIFICATE OF COMPLETION = ZERO MARKS

Attach a copy or image of your Faculty of Health HSE Induction Certificate of Completion

HERE

SUBTOTAL:

Working Safely in the Laboratory – HSE Assignment

(2 marks)

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2.

LQB180 Foundations of Biochemistry

Safety Features and Safety Practices in the Laboratory

Different laboratories will have different layouts and settings, but in order to operate as a laboratory they need to comply with standard safety rules and regulations and incorporate critical safety equipment. We have reviewed the operating design and layout of the Q4 laboratory in the LQB180 tutorial sessions and a comprehensive plan of the Q4 laboratory is included in your notes. •

In the table below list four (4) key safety features that are important for general student practical work that is conducted in a PC2 – compliant laboratory facility, such as the Q4 laboratory.

•

In addition, provide an explanation (1 – 2 concise and brief sentences) of the function and purpose of each safety feature.

Q4 Safety Feature

Explanation of Safety Feature Function / Purpose

SUBTOTAL:

(4 marks)

Marking: each correct answer (safety feature + explanation) will be awarded 1 mark

Working Safely in the Laboratory – HSE Assignment

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Faculty of Health

LQB180 Foundations of Biochemistry

3. Chemicals and Solutions – safe handling procedures (a) Understanding Hazardous Chemical Labels A hazardous chemical label is a group of written, printed or graphical information elements about a hazardous chemical that is affixed to, printed on or attached to the container. In 2012 Work Health and Safety (WHS) Regulations introduced a new system of labelling for hazardous chemicals based on the United Nations’ Globally Harmonised System (GHS) of Classification and Labelling of Chemicals. Pictograms are graphic images that immediately show you what type of hazard a hazardous product presents. Manufacturers and suppliers classify the products into one or more of the hazard classes and assign one or more of the appropriate pictograms. Pictograms will be on the product supplier labels of the hazardous products as well as on the safety data sheets (SDSs). Workers must gain familiarity with the ten hazard pictograms and know what they mean. Most pictograms have a distinctive red border in the shape of a square set on one of its corners. Inside this border is a symbol that represents the potential hazard (e.g., fire, health hazard, corrosive, etc.). With a quick glance, a worker can see, for example, that the product is flammable or if it might be a health hazard. Using the set of hazard pictograms [a.  i.] shown below, match the correct hazard description [i)  ix)] in the table to the proper hazard pictogram [a.  i.].

a.

b.

c.

d.

f.

g.

h.

i.

Working Safely in the Laboratory – HSE Assignment

e.

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LQB180 Foundations of Biochemistry

Pictogram

Description

(choose correct letter)

i)

Gases under pressure

ii)

Fire hazards

iii)

Oxidizing hazards

iv)

Can cause death or immediate and serious toxic effects May cause less serious health effects or damage the ozone layer Organisms or toxins that can cause diseases in people or animals Can cause corrosive damage to metals, as well as skin and eyes

v) vi) vii) viii)

Explosion or reactivity hazards

ix)

Serious health effects

SUBTOTAL:

(3 marks)

Marking: each incorrect answer will be deducted 0.5 marks

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LQB180 Foundations of Biochemistry

(b) Safety Data Sheets (SDS) and Chemwatch For every chemical or solution used in the laboratory there is a Safety Data Sheet (commonly referred to as a SDS) available that provides comprehensive information related to the chemical or solution. SDS information can be readily accessed through a database called Chemwatch, which is available on many QUT computers. A full comprehensive SDS and a summary or mini SDS are available for each chemical / substance. Working individually using the attached PowerPoint slide handout as a guide, open the Chemwatch program on the computers (QUT Library or Computer Labs) and find the full SDS for 1 M sodium hydroxide solution [1 M NaOH is equivalent to 4% w/v NaOH]. Note: NaOH is a common base (alkali) used frequently in biomedical laboratories. ‘M’ refers to ‘molar’ or moles/litre and is a measure of concentration. In this case there are 1 moles NaOH per litre of solution. Percentage weight / volume (% w/v) is also a concentration and for 1 M NaOH the equivalent % value will be 4% (w/v), which will be 4 g per 100 mL (40 g per litre). The information in the SDS is set out under sixteen separate sections, which provide details about risks and safety procedures that must be applied when working with the chemical. NOTE: Chemwatch will list a very large number of options for sodium hydroxide solutions with a variety of concentrations. •

Input the chemical name as ‘1M sodium hydroxide’ o You should see a relevant match in the top 5 options.

•

Remember: Chemwatch assigns each chemical a Chemwatch Number (CW number) in the database system – each CW number is searchable and displays an exact match for the chemicals SDS.

Ensure that you locate a SDS and mini SDS for 1 M (mol/L) NaOH • Double-check that you have the correct SDS • Does the information at the top of the SDS clearly state the correct molarity? • Does the information at the top of the SDS clearly state the correct % value? An SDS that complies with the WHS Regulations contains 16 separate sections each with specific information relating to the chemical being used, handled, stored, transported or disposed. Working Safely in the Laboratory – HSE Assignment

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LQB180 Foundations of Biochemistry

Complete the table below listing the section titles (by heading) for each of the 16 sections.

Table 2. Contents of the Safety Data Sheet (SDS) – for example, Sodium Hydroxide 1 M. Section Number

Section Title Heading

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

SUBTOTAL:

(2 marks)

Marking: deduct 0.5 mark for each incorrect / missing section heading

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Faculty of Health

LQB180 Foundations of Biochemistry

The Mini SDS summarises the key information required for work practice and is used routinely in laboratories. The information on the Mini SDS allows for the safe preparation, handling, storage and disposal of substances and provides the current clean-up and/ or first aid protocol to be followed if there is a spill or an accident involving human contact or ingestion.

Print off AND attach a copy of the Mini SDS for 1 M sodium hydroxide (NaOH) to your HSE Assignment. NB: Ensure that you print off and attach the correct Mini SDS for 1 M (mol/L) NaOH, which as a percentage value will be 4% (w/v).

SUBTOTAL:

Working Safely in the Laboratory – HSE Assignment

(2 marks)

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LQB180 Foundations of Biochemistry

Answer the following questions using the information for 1 M NaOH available from the Chemwatch website (including Gold SDS, Mini SDS and Emergency Report). 1. What is the key property of sodium hydroxide (as identified in the GHS hazard pictogram) that makes it hazardous? .............................................................................................................................................. Select the GHS pictogram that is applicable for 1 M sodium hydroxide (add or drag and drop a tick box  underneath the box you select).

2. When carrying out an experiment, one of your fellow students has accidentally spilt a solution of 1 M sodium hydroxide and a small volume has splashed onto their face. Fortunately, their eyes were protected by safety glasses, but the solution has splashed onto their cheek. What first aid measures should be taken? .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. ..............................................................................................................................................

NOTE: Refer to Chemwatch Emergency Report for First Aid and Spills information

SUBTOTAL:

Working Safely in the Laboratory – HSE Assignment

(2 marks)

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Faculty of Health

LQB180 Foundations of Biochemistry

(c) Labelling solutions In preparation of solutions, the most important health and safety information can also be included on the labels attached to the solution bottles. These labels can be created using the Chemwatch database.

Below, you an example of a label for a solution of sodium hydroxide (1 M).

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LQB180 Foundations of Biochemistry

In the spaces below list the Risk Phrases and Safety Phrases associated with use of this hazardous chemical solution. Risk Phrases .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. ..............................................................................................................................................

Safety Phrases .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. ..............................................................................................................................................

SUBTOTAL:

(2 marks)

Marking: Deduct 0.5 marks for each POINT that is missing Working Safely in the Laboratory – HSE Assignment

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LQB180 Foundations of Biochemistry

Laboratory Biosafety Classification

Laboratory facilities are rated according the the types of laboratory work, risks and levels of containment for potentially infectious agents. At the lowest level of laboratory biosafety, precautions may consist of regular hand-washing and minimal protective equipment. At higher biosafety levels, precautions may include airflow systems, multiple containment rooms, sealed containers, positive pressure personnel suits, established protocols for all procedures, extensive personnel training, and high levels of security to control access to the facility. In Australia the laboratory biosafely rating system conforms to the Australian/New Zealand Standards (AS/NZS 2243.3: 2010—Safety in laboratories - microbiological safety and containment). This recognises a number of levels of laboratory biosafety risk that are designated ‘PC’ levels. The laboratories (Q4) you are working in are classed as PC2 level laboratories.

What does ‘PC’ stand for and how many PC biosafety levels are recognised? .............................................................................................................................................. .............................................................................................................................................. Provide a brief description of what PC2 means in the context of laboratory biosafety? .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. .............................................................................................................................................. ..............................................................................................................................................

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LQB180 Foundations of Biochemistry

Name an infectious or pathological agent that can only be investigated in laboratories with the highest PC biosafety level accreditation. .....................................................................................................