Probset 04 - Mandatory assignment for Wayne Forrester\'s genetic class, to be completed weekly PDF

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Mandatory assignment for Wayne Forrester's genetic class, to be completed weekly...

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Pr obl e m Se t4, F a l l2 0 1 7 8Poi nt sT ot a l

S t ude ntNa me :

1 . Ac r os sbe t we e napur e br e dbl a c kmous ea ndapur e br e dwhi t emous eyi e l dsa l lbl ac kF1offs pr i ng. The s ebl a c kF1s i bl i ngmi c ea r ec r os s e dt opr oduc ea nF2ge ne r a t i on. Youobs e r vet hef ol l owi ngF2 phe not ypi cr a t i o: Whi t e :2 2 Bl a c k: 1 7 5 Gr a y:1 1 4 a .Dr a w outt hec r os s e sde s c r i be da bove , s howi ngt hege not ype soft hepa r e nt s , F1a ndF2.

b . Youc r os swhi t emi c ef r om t heF2ge ne r a t i onXbl a c kmi c ef r om t heF2ge ne r a t i on. Wha ti st he pr oba bi l i t yofge t t i ngbl a c koffs pr i ngf r om t hi sc r os s ?

2 .Youc r os sapur e br e dr e de ye dmous ewi t hapur e br e dye l l o we ye dmous ea ndy ouge ta l lr e de ye d F1s .Whe ny out he ns e l ft heF1syouge ta nF2ge ne r a t i onc ompos e doft hef ol l owi ng: 1 7ye l l o we ye s , 2 0 7r e de ye s , a nd5 0bl uee ye s . a .Gi vet hege not ype sofF1a ndF2;i . e . dr a w outt hec r os s .

b .Wha ti st hes i mpl i fie dr a t i o? c .How doe st hi sr a t i oar i s e ?

3 . A ma l ef r ui t flywi t hc ur l ywi ngsi sc r os s e dt oaf e ma l ewi t hnor ma lwi ngs . Youne xtc r os sc ur l y ma l eF1' sXc ur l yf e ma l eF1' st oyi e l dt heF2' ss hownbe l o w. F1: ( f r om c ur l ywi ngmal eX F2( f r om c ur l yma l eF1X nor ma lwi ngf e mal e ) mal e sc ur l yf e ma l eF1 ) : 1/4nor ma lma l e s 1 /3c ur l y ma l e s 1/4nor ma lf e mal e s 1 /3c ur l yf e ma l e s 1/4c ur l yma l e s 1 /6nor ma lma l e s 1/4c ur l yf e ma l e s 1/6nor ma lf e mal e s a .Di a gr a mt hec r os sofF1’ st ha tpr oduc e dt heF2pr oge nys ho wn. Al s os how t hege not ype sof t heF1' sandF2' s . De finey ourc hoi c eofs ymbol s .

1

Pr obl e m Se t4 , Fa l l2 0 1 7 8Poi nt sT ot a l

S t ude ntName :

b . Sugge s tamodeofi nhe r i t a nc et ha te x pl a i nst hea bover a t i os( i . e . domi na nt ,r e c e s s i ve ,x l i nke d, a ut os oma l , e t c . )

4 . The r ea r et wona t ur a l l yoc c ur r i ngs t r a i nsoft heoc t opus ,Z. c r a b t r e e a e , t hos ewi t hs hor tt e nt a c l e sa nd bl ues pot sa ndt hos ewi t hl ongt e nt a c l e sandy e l l ow s pot s . Youc r os sat r ue br e e di ngs hor tt e nt a c l e , bl ues pot t e doc t opusa ndat r ue br e e di ngl ongt e nt a c l e d,ye l l o ws pot t e doc t opus . TheF1’ sal lha ve l ongt e nt a c l e sa ndgr e e ns pot s . Youc r os st heF1’ sa mongs tt he ms e l ve sa ndfindt hef ol l o wi ng ’ F s : 2 3 /16l ongt e nt ac l e , ye l l ow s pot t e d 1 /16s hor tt e nt a c l e , ye l l ow s pot t e d 6 /16l ongt e nt ac l e , gr e e ns pot t e d 2 /16s hor tt e nt a c l e , gr e e ns pot t e d 3 /16l ongt e nt ac l e , bl ues pot t e d 1 /16s hor tt e nt a c l e , bl ues pot t e d a .Wha ti st hege not ypeofe a c hoft heF2pr oge ny?

b . I fyouc r os ss hor tt e nt a c l e , bl ues pot t e dF2b ys hor tt e nt a c l e , gr e e ns pot t e dF2, wha twi l lt he offs pr i ngl ookl i ke ?

5 . I nt hej ungl e sofJ ama i c al i ve sac ol or f uls pe c i e sofbe e t l e ,C. dr a k e a e . Thr e es t r a i nsofC. dr a k e a ea r e known. S t r a i n1ha sr e dwi ngc ove r s , s t r a i n2ha sor a ngewi ngc o ve r sa nds t r a i n3ha sgr e e nwi ng c ove r s . Yout a keme mbe r sofe a c hs t r a i nba c kt ot hel a ba ndpe r f or mt hef ol l owi ngc r os s e s , whi c h yi e l dt her e s ul t ss hown. s t r a i n1Xs t r a i n2 :a l lgr e e n s t r a i n1Xs t r a i n3 :1 /2r e da nd1 /2gr e e n s t r a i n2Xs t r a i n3 :1 /2or a ngea nd1 /2gr e e n a .Wha ta r et hege not ype soft het hr e es t r a i ns ? s t r a i n1_ _ __ _ _ s t r a i n2_ _ __ _ _ s t r a i n3_ _ __ _ _ 2

Pr obl e m Se t4 , Fa l l2 0 1 7 8Poi nt sT ot a l

S t ude ntName :

b . Wha tphe not ype sdoyoue x pe c tf r om ac r os sofs t r a i n3Xs t r a i n3 ?Pl e a s ei nc l udet he r e l a t i vef r e que nc i e sofe a c hphe not ype .

6 . Youdi s c ove rane ws ubs t a nc e( Subs t anc eE)t ha ti si nvol ve di nt hepr oduc t i onofz i gz a gs t r i pe s . Youa r ei nt e r e s t e di nunde r s t a ndi ngt hege ne t i cbas i sf ors ubs t a nc eEs ynt he s i s , a ndt he r e f or eus e da ge ne t i cs c r e e nt oi de nt i f y8mut a t i ons( numbe r e d1–8 )t ha tpr e ve nts ubs t a nc eEs ynt he s i s . Youc r os s homoz ygousr e c e s s i vepa r e nt si nal lpos s i bl epa i r wi s ec ombi na t i onst oge ne r a t et her e s ul t spr e s e nt e d i nt het abl e . 1 2 3 4 5 6 7 8

1 + + + + +

2 + + + + + -

3 + + + + +

4 + + + + +

5 + + + + + + +

6 + + + + + -

7 + + + + + + +

8 + + + + + -

a .Wha ti st hena mef ort hi st ypeofc r os sandwha tt ypeofi nf or ma t i ondoe si tpr ovi de ?

b .How ma nyge ne sa r ei nvol ve di nt hepr oduc t i onofSubs t a nc eE?

c .Show whi c hmut a t i onsbe l ongt oe a c hge ne , andgi vee a c hge neas ymbol .

d.Shownbe l ow i st hebi oc he mi c alpa t hwa yl e a di ngt opr oduc t i onofs ubs t a nc eE. Youa s s a yf or e a c hoft hei nt e r me di a t e si ne a c hoft hee i ghtmut ant s . A +me a nst ha tt hec ompoundwa sde t e c t e d i nmut a ntc e l l swhe r e asa–i ndi c a t e st ha ti twa snot . Wr i t ea bovee a c ha r r o wt hege ne ( s )t ha t e nc odet hee nz yme ( s )r e s pons i bl ef ort ha ts t e pofs ubs t a nc eEs ynt he s i s . mut a nt s 1 2 3 4 5 6 7 8 A + + + + + + + + B + - + + + - + C + - + + + - - D - - - - + - - 3

Pr obl e m Se t4 , Fa l l2 0 1 7 8Poi nt sT ot a l E

S t ude ntName :

- - - - - - - A

B

C

D

Substance E

7 . Dur i ngyouri nve s t i ga t i onsoff r ogs pe c i e si nCos t aRi c ayoufindt wos t r a i nsoft hes pl e ndi dpoi s on da r tS.y d e a e . S t r a i n1ha sye l l ow bodi e sa ndr e de ye s . S t r a i nt woha sbl uebodi e sa ndwhi t ee ye s . You c r os ss t r ai n1f e ma l e swi t hs t r a i n2mal e s . Ther e s ul t i ngF1' sa l lha vegr e e nbodi e sa ndr e de ye s . You c r os sF1' st opr oduc et hef ol l owi ngF2' sa tt hef r e que nc i e si ndi c a t e d: 1/4gr e e nbodi e d,r e de ye df e ma l e s 1/8ye l l owbodi e d, r e de ye df e ma l e s 1/8bl ue bodi e d, r e de ye df e ma l e s 1/8gr e e nbodi e d,r e de ye dmal e s 1/8gr e e nbodi e d,whi t e e ye dma l e s 1/1 6ye l l owbodi e d, r e de ye dma l e s 1/1 6ye l l owbodi e d, whi t e e ye dmal e s 1/1 6bl ue bodi e d,r e de ye dmal e s 1/1 6bl ue bodi e d,whi t e e ye dma l e s a . Pl e a s egi vet hege not ype soft heF2a ni ma l si nt hes pac epr ovi de d.

b . Pl e a s egi vet hege not ype s , phe not ype sande x pe c t e df r e que nc i e soft hedi ffe r e ntc l a s s e sof offs pr i ngt ha twoul dr e s ul tf r om ac r os sofgr e e nbodi e d, r e de ye dma l e sXy e l l o wbodi e d, r e d e ye d-f e mal e s .

8 . Whi l er e s e a r c hi ngme t hodsofye ar r oundf a r mi ng, youi s ol a t e6i nde pe nde ntmut a t i onst ha ta l l ow s oybe a nst owi t hs t a ndf r e e z i ng . Al l6 l i ne swe r ei nt e r c r os s e d, a ndy ouobt ai n 1 2 3 4 5 6 t hef ol l o wi ngr e s ul t s( +i ndi c a t e swi l d 1 – + + – + + t ypepl a nt s , –i ndi c a t e sc ol dr e s i s t a nt ) : 2 3 4 54 6

+ + – + +

– + + + +

+ – + – +

+ + – + +

+ – + – +

+ + + + –

Pr obl e m Se t4 , Fa l l2 0 1 7 8Poi nt sT ot a l

S t ude ntName :

A. Wha ti st hena meoft hi st ypeoft e s t ?

B. Ho w manyi ndi vi dua lge ne swe r ei de nt i fie d?Whi c hmut a t i onsar ea l l e l i c ?

C. I nf ur t he rs t udi e s , mut a t i on# 7i sa l s of oundt oc a us er e s i s t a nc et of r e e z i ng . I ti sde t e r mi ne d t ha tmut a t i ons# 2a nd7ar ea l l e l i c . Addt hec ompl e me nt a t i ont e s tr e s ul t si nt hes pa c epr ovi de d i nt hec ha r ta bove .

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