Salisa de la pena la vida loca la mi syan PDF

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IMMUNOLOGY-SEROLOGY VIRTUAL LABORATORY WORKSHEET ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) INSTRUCTION: Go to http://www.hhmi.org/biointeractive/immunology-virtual-lab. Start the Virtual Lab and maximize the screen if you wish. Answer the following questions in the spaces provided.

GUIDE QUESTIONS: 1. Where are antibodies found? - The antibodies are found in the plasma of the blood. 2. How can they be used in the laboratory? - It determines whether the patient carries a disease in the sample. 3. What does ELISA stand for? - It stands for Enzyme-linked Immunosorbent Assay. 4. What are ELISA assays used for in the laboratory? - It measures the concentration of antibodies or antigens in the presented sample. 5. What are the three important limitations of an ELISA? Explain each. Limitations Explanations

test is able to detect antibodies but not enough to confirm that the patient is sick?

tendecies to show false negative results?

unrelated antibody may react with the antigen nonspecifically which a positive result may arise.

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Ann Brokaw

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Ann Brokaw

BACKGROUND 1. What test can be used to determine whether a patient has an infectious or autoimmune disease? - The Glisa test is used to determine whether a patient has an infectious or autoimmune disease. 2. What does a positive result indicate? - Because of the confirmed presence of antibodies, it implies that the patient has encountered a diesease. 3. The watery fluid of the blood is called serum. 4. What is allowed to react with the target antigen? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 5. Detection is possible when ______________________________________________________ 6. Once isolated, the secondary antibody can be _______________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 7. What is the signaling system? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 8. What happens when the appropriate chemical substrate is added? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 9. How is the test quantified? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________ 10. What does the amount of color reflect? ______________________________________________________________________________ ______________________________________________________________________________ ______________________________________________________________________________

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Ann Brokaw...