Review Test Submission Quiz 3 - Cell Culture & Western Blot & PDF

Preview

Summary

quiz...

Description

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

myBSU

Jodyann Munroe

H

Course Content ... Lab 5 - Cell Signaling and Western Blot I - Background Information Study Review Test Submission: Quiz 3 - Cell Culture & Western Blot

Review Test Submission: Quiz 3 - Cell Culture & Western Blot

User

Jodyann Munroe

Course

BIOL 200L-A01 Cell Biology (LAB) Su20

Test

Quiz 3 - Cell Culture & Western Blot

Started

6/21/20 11:44 PM

Submitted

6/22/20 12:50 AM

Due Date

6/21/20 11:59 PM

Status

Needs Grading

Attempt Score

0 out of 20 points

Time Elapsed

1 hour, 6 minutes

LATE

Results Displayed Submitted Answers, Correct Answers

Question 1

1 out of 1 points

Why is nitrocellulose membrane useful in the Western blot technique? Selected Answer:

Correct Answer:

It binds proteins nonspecifically and very tightly so that proteins become immobilized on the membrane during electroblotting. It binds proteins nonspecifically and very tightly so that proteins become immobilized on the membrane during electroblotting.

Question 2

1 out of 1 points

During Western blot, the secondary antibody binds directly/specifically to the protein being investigated. Selected Answer: Correct Answer:

Question 3

False False

1 out of 1 points

Which of the following would qualify as a mammalian cell culture? https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 1/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

Selected Answer:

Correct Answer:

Cells from a human tumor biopsy that were isolated and placed on a tissue culture dish. Cells from a human tumor biopsy that were isolated and placed on a tissue culture dish.

Question 4

1 out of 1 points

Antibodies are: Selected Answer:

proteins produced by the immune system that bind a specific antigen.

Correct Answer:

proteins produced by the immune system that bind a specific antigen.

Question 5

1 out of 1 points

Why is the inclusion of a protein standard important when conducting SDSPAGE and Western blot? Selected Answer:

Correct Answer:

It allows you to estimate where proteins of a given size should be located on the gel and then membrane. It allows you to estimate where proteins of a given size should be located on the gel and then membrane.

Question 6

1 out of 1 points

Place the steps below in the correct sequence if you wanted to conduct Western blot analysis on PC12 cells growing in culture. Correct Answer 1.

Harvest PC12 cell lysates using a lysis buffer containing SDS (e.g., Laemmli buffer) 2.

Perform SDS polyacrylalmide gel electrophoresis (SDS-PAGE)

Selected Answer 1.

Harvest PC12 cell lysates using a lysis buffer containing SDS (e.g., Laemmli buffer) 2.

Perform SDS polyacrylalmide gel electrophoresis (SDS-PAGE)

https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 2/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

3.

3.

Transfer the protein onto a nitrocellulose membrane 4.

Transfer the protein onto a nitrocellulose membrane 4.

Probe the nitrocellulose membrane with an antibody that binds your protein of interest

Probe the nitrocellulose membrane with an antibody that binds your protein of interest

Question 7

1 out of 1 points

What role does Trypan blue play during cell counting?

Selected Answer:

It enables you to distinguish live cells versus dead cells.

Correct Answer:

It enables you to distinguish live cells versus dead cells.

Question 8

1 out of 1 points

Electroblotting refers to the process by which proteins are: Selected Answer:

Correct Answer:

Question 9

transferred from an SDS-PAGE gel to a nitrocellulose membrane. transferred from an SDS-PAGE gel to a nitrocellulose membrane.

1 out of 1 points

While conducting a Western blot, if you did not treat your membrane with a blocking buffer before incubating with the primary antibody, what would be the consequence? Selected Answer:

Correct Answer:

Question 10

The antibody would stick non-specifically to the membrane, rather than only to its antigen. The antibody would stick non-specifically to the membrane, rather than only to its antigen.

1 out of 1 points

https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 3/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

Why are cell lysates exposed to SDS and high heat before subjecting them to SDS-PAGE? Selected Answer:

It causes denaturation of the proteins within the lysate.

Correct Answer:

It causes denaturation of the proteins within the lysate.

Question 11

1 out of 1 points

In the final step of Western blot analysis, why is the molecule luminol added to the membrane/blot? Selected Answer:

Correct Answer:

HRP conjugated to the secondary antibody breaks down luminol, resulting in the emission of light. HRP conjugated to the secondary antibody breaks down luminol, resulting in the emission of light.

Question 12

1 out of 1 points

During SDS-PAGE (SDS-polyacrylamide gel electrophoresis): Selected Answer:

Correct Answer:

Question 13

proteins in a mixture are separated in a gel based on their relative size. proteins in a mixture are separated in a gel based on their relative size.

1 out of 1 points

During Western blot, antibodies that are conjugated to horseradish peroxidase (HRP) are commonly used because: Selected Answer:

Correct Answer:

Question 14

when incubated with its substrate luminol, it emits light that can be visualized. when incubated with its substrate luminol, it emits light that can be visualized.

0 out of 1 points

https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 4/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

After this week’s review of background information on cell culture and Western blot, you will go through a virtual experiment investigating whether NGF (select ALL correct answers): Selected Answers:

causes up-regulation/increased expression of Egr1 in PC12 cells.

Correct Answers:

causes up-regulation/increased expression of Egr1 in PC12 cells. causes neurite outgrowth in PC12 cells.

Question 15

1 out of 1 points

What is a hemocytometer? Selected Answer:

Correct Answer:

Question 16

A device used to count cells and determine the cell concentration within a cell suspension. A device used to count cells and determine the cell concentration within a cell suspension.

1 out of 1 points

You need to determine the cell concentration of a cell suspension. To do so, you dilute you cell suspension 1:1 with Typan blue, pipet the suspension on a hemocytometer, view the hemocytometer under a microscope, and count the number of cells within the 5 red squares highlighted below. Based on your cell count, what is the concentration of LIVE cells in your suspension? NOTE: The equation needed to convert your cell count into cell/ml is included in the image below.

https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 5/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

Selected Answer:

364,000

Correct Answer:

364,000

Question 17

1 out of 1 points

When growing mammalian cells in culture, why is it critical to grow them in a solution called cell culture medium? ANSWER: Because the cell culture medium: Selected Answer: https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 6/7

2/24/2021

Review Test Submission: Quiz 3 - Cell Culture & Western Blot &...

provides the cells with glucose, vitamins, and other nutrients that they need to live and proliferate. Correct Answer:

provides the cells with glucose, vitamins, and other nutrients that they need to live and proliferate.

Question 18

0 out of 1 points

What does the term epitope refer to? Selected Answer:

Correct Answer:

The region of antibodies that varies in their amino acid sequences. The specific region on an antigen to which an antibody binds.

Question 19

1 out of 1 points

During Western blot, the primary antibody binds directly/specifically to the protein being investigated. Selected Answer: Correct Answer:

True True

Question 20

1 out of 1 points

What units are typically used to refer to protein size? Selected Answer:

kDa (kilodaltons)

Correct Answer:

kDa (kilodaltons)

Wednesday, February 24, 2021 10:04:03 AM EST

← OK

https://bridgew.blackboard.com/webapps/assessment/review/review.jsp?attempt_id=_7170032_1&course_id=_68837_1&content_id=_2541757_1&out… 7/7...