Quiz 1 - Notes on lab quiz 1 PDF

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Notes on lab quiz 1...

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Quiz 1  It asked to draw glutamine, asked what the purpose of the lab was and how you'd do it, name the two amino acids that have S in them, name the two amino acid isomers, and label a diagram (figure 1.3 in lab)  it also asks to name the general structures all amino acids have (amide group, carboxylate group, r chain)  it also asks what would bind to an anion-exchange, which would be a negative amino acid  1. Name 3 positively charged amino acids  2. What is the goal of this lab and how do we find it  3. What is a cation and anion  4. Label the tube (there is a pic in the manual of it)  5. What macromolecule is made up of amino acids Quiz 2  So I had a quiz today, but you had to draw out the aspartyl-alanine peptide bond, state what the variables in the beer lambert law are and what they represent, whether or not absorbance had units, and why a microplate reader is better than a spectrometer  I had a question that asked what the wavelength was to be able to see for the human eye  i had mine today! it was: explain the purpose of the lab, what is the beer-lambert law and its units, draw glycyl-alanine dipeptide, and how does gel filtration work Quiz 3  Ours asked what Km and Vmax were, what the active unit for LDH is, what reaction we were carrying out, why we studied this reaction and not the reverse in lab, what wavelength we were using and why, and to draw a general michaelis-menten graph. Hope this helps  Km: substrate concentration at which half the enzyme active sites are filled  Vmax: rate of reaction when all active sites are filled by substrate  1 enzyme activity unit for LDH = amount of enzyme that will reduce 1micromole of pyruvate to lactate per minute at neatral ph and room temperature (ph7 25C)  Carrying out the conversion of pyruvate to lactate imitating a step of anaerobic glycolysis.  We study this and not reverse because the reverse has positive delta G (positive gibbs free energy) so its not spontaneous like the forward reaction.  we are using 340nm because NADH absorbs this wavelength well and NAD+ doesnt and equal moles of pyruvate converted to lactate are converted from NADH to NAD+ so it can be used as a progress tracker for the reaction.  Mine asked what kind of enzyme lactase was  Also, the standard temperature for an enzyme is 37, not 25 as was stated below that other post Lab 4  the lab quiz i had was

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1. purpose of the lab 2. write the equation for first step of Wij's reaction 3. define iodine number 4. Differences between cis and trans 5. where is fat stored in the body 6. Unsaturated makes _____ membranes and saturated makes ______ membranes 7. Cause of atherosclerosis 8. How can one be healthier in relation to polyunsaturates/saturates 1. The purpose is to determine the iodine number of a lipid sample, used to determine its degree of unsaturation and gives the ability to determine if the sample is a fat or oil. 2. HC=CH + ICl ---> CHI-CHCl The ICl solution reacts with the double bonds in the unsaturated fatty acid to form di-halogenated single bonds. 3. The amount of iodine in grams that is consumed by 100g of the lipid. 4. Trans has hydrogens on opposite sides of the double bond and is man made. cis has hydrogens on the same side as the double bond and occurs naturally. Trans: -CvH=C^H- Cis: -C^H=C^H5. I think the answer they were looking for is "in adipose or fatty tissue" 6. Unsaturated makes "Fluid-like" membranes and saturated makes "gel-like" membranes 7. Too much saturated fats hardening arteries 8. increase plant consumption and decrease meat consumption asked for the difference between cis and trans fatty acids (asked for 2 differences)

Quiz 5                

- standard carb formula - 2 types of disaccharides - the organ that uses glucose for fuel (brain) - glucose storage for animals - glucose storage for plants - glucose cell that is in plant wall and type of bond (cellulose and B-1-4 linkage) 1. (CH2O)n 2. Lactose and Sucrose 3. The brain 4. Glycogen (do you mean the type of polysaccharide its stored as?) 5. Starch (same note as above) 6. Cellulose connected by B-1,4 glycosidic links They also ask the functions of polysaccharides: energy storage & structure strength 1. What is the purpose of today's lab? 2. Name two monosaccharides 3. Is lactose a reducing sugar? Why?

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4. What is the polysaccharide storage in plants? 5. What is the Maillard reaction? 6. What is the structure of polysaccharides in plants and how are they linked?...